Microscopy

Updated 14/12/2004

Laboratory


Research Inst.


Teaching Inst.


University

You'll find here some things I posted on for my students. Provided you find it useful you can freely use them, just acknowledge please !
General	Contents	Optical lens principle	Thin lens principle	Spherical aberration	Chromatic aberrations
	Image of a point Airy diffraction	Resolution	Numerical Aperture	Resolving power of an objective	Bi-refringence
		Cauchy law	Bragg law	Human eye
Photonic microscopy	Principle	321 Huygens ocular
Polarised-light microscopy	Principle	Microquartz	Twins and clivage in olivine
Dark-field microscopy	Principle	Spermatozoids
Phase-contrast microscopy	Principle	Spermatozoids in positive phase contrast	Spermatozoids in negative phase contrast	Spermatozoids in interference phase contrast
Fluorescence microscopy	Principle	Grafted BSA molecule
Confocal microscopy	Principle	Comparison normal/confocal	with LASER scanning	Granite craks	Protein location in a cell
Holographic microscopy	Principle	Neuron	Fresnel lens	In color levels
Electron microscopy	Electron-Matter interaction	Diffusion	The emmissive "pear"	Electron distribution versus energy
Scanning Electron Microscopy (SEM)	Principle	A SEM eqquipment	Indus pigments	their EDX diagram	Topographic image of Ni single crystal
		Secondary vs backscattered image	Nervous cell in backscattering mode	Fe-EDX image of a Fe-Ti alloy	Ti-EDX image of a Fe-Ti alloy
	K-EDX image of a Fe-Ti alloy	Chemical attack of an InP single crystal
Electron BackScattering Diffraction (EBSD)	Principle	Kikuchi bands formation	Orientation Mapping principle	Bands detection	Bands Indexing
	Calcite EBSD pattern	Omphacite EBSD pattern		Grain orientation map of calcite	Calcite misorientation grain distribution
Transmission Electron Microscopy (TEM)	Principle	TEM cross sectionned instrument	Electron Gun	Image/diffraction modes	Dislocations in GaN
	Al precipitates	High resolution image of a silicate	GaN on saphire substrate	Body centred (I) Diffraction	High Resolution negative image
	Fourier Transformed	+ background filtering	+ TF-1
Convergent Beam Electron Diffraction	Principle	Defocused Convergent Beam	Typical diffraction image	Si example	GaAs example
Near Field Microscopy (AFM, Tunnel ...)	Principle	Scanning monitoring	LiNbO3 AFM image	Optical Tunneling principle	Optic Fiber system
	Functionnalised mode	Different Scanning modes	Magnetic Force image of Co	Molecular Conformation	Living cell viewed in different modes
Exams	January 2004	Correction